發展檢測SARS病毒引物的研究機構及7對target序列
發展檢測SARS病毒引物的研究機構及7對引物序列

Bernhard-Nocht Institute
Bernhard-Nocht Str. 74
D-20359 Hamburg
Germany

BNIoutS/BNoutAs: sense ATGAAT TAC CAA GTC AAT GGT TAC
antisense CAT AAC CAG TCG GTA CAG CTA C
FraGment lenGth 195 bp

BNIinS/BNIAs: sense GAA GCT ATT CGT CAC GTT CG
antisense CTGTAGAAA ATC CTA GCT GGA G
Fragment length 110 bp

SAR1s/SAR1as: sense CCT CTC TTG TTC TTG CTC GCA
antisense TAT AGT GAG CCG CCA CAC ATG
Fragment length 150 bp

Amplicon sequence (BNI-1, Bernhard-Nocht Institute, Hamburg, Germany)
TACCGTAGACTCATCTCTATGATGGGTTTCAAAATGAATTACCA
AGTCAATGGTTACCCTAATATGTTTATCACCCGCGAAGAAGCTAT
TCGTCACGTTCGTGCGTGGATTGGCTTTGATGTAGAGGGCTGTCAT
GCAACTAGAGATGCTGTGGGTACTAACCTACCTCTCCAGCTAGGA
TTTTCTACAGGTGTTAACTTAGTAGCTGTACCGACTGGTTATGTTGA
CACTGAAAATAACACAGAATTCACCAGAGTTAATGCAAAACCTCC
ACCAGGTGACCAGTTTAAACATCTTATACC (擴增產物在基因組上的位置︰18,105......18,406)

Centers for Disease Control & Prevention
National Centers for Infectious Diseases,
Atlanta GA 30333
1600 Clifton Road, NE, MS-C12
United States
IN-2,IN-4: sense 5' GGGTTGGGACTATCCTAAGTGTGA 3'
antisense 5' TAACACACAAACACCATCATCA 3'
Expected product size: 452 bp

IN-6,IN-7: sense 5' GGTTGGGACTATCCTAAGTGTGA 3'
antisense 5' CCATCATCAGATAGAATCATCATA 3'
Expected product size 440 bp





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